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ATP content and viability of spermatozoa drive variability of fertilization success in the Pacific oyster ( Crassostrea gigas ) ArchiMer
Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc.
Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability...
Tipo: Text Palavras-chave: Spermatozoa; Quality; Motility; Viability; ATP content; ROS production.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00386/49769/50309.pdf
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